Abstract

MODULATORY EFFECTS OF LNM ON LYMPHOCYTIC APOPTOTIC PATHWAY THROUGH P53, BCL2,

Hassani. H.H, AL-Tamimi. I.A., AL-Taie L.H., Kadhim T.A.*

ABSTRACT

By using immunocytochemistry technique the apoptotic activity of Leinamycin against lymphocytes isolated from Acute Lymphocytic Leukemia patients in comparison with healthy individuals was determined by measuring P53 and Bcl-2, were they play very important role in cell cycle and then programed cell death.Results showed that LNM at concentration 4.5 μg /ml caused significant upregulation of P53 while it decreased the expression level of the anti-apoptotic protein Bcl-2 in lymphocyte. The results showed significantly (P< 0.001) improvement of P53 expression level in treated cells (43.363 ±1.893%) when compared with untreated cells (14.371 ± 0.449 %), Whereas, LNM caused dramatic decreasing in Bcl-2 expression pattern when compare with untreated cells (30.08± 1.0095, 63.2± 1.43; P < 0.001) respectively.

Keywords: P53, BCL-2, Leukemia, programed cell death, Leinamycin.