Photo Gallery

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from  7.454 to 7.632  due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • WJPPS: SEPTEMBER ISSUE PUBLISHED
  • SEPTEMBER 2021 Issue has been successfully launched on 1 September 2021.

Abstract

IN-VITRO EVALUATE ANTAGONISM STRAINS OF ACTINOMYCETES ON GROWTH STRAINS OF RHIZOBIUM

Abdouslam M. Rassem* and Arun A. David

ABSTRACT

The work evaluates the antagonism between strains of Actinomycetes and strains of Rhizobium, soil samples were collected from field pea nodules area, actinomycetes and rhizobium strains were isolated and cultured on selective media, three strains of both actinomycetes and rhizobium were isolated, identified using morphology and biochemical properties, the isolates identification was confirmed by molecular method of 16S rRNA gene amplification. Thus the six isolates were identified as rhizobium (RS1-leguminosarum, RS2- Sinorhizobium meliloti and RS3-Rhizobium pusense) and actinomycetes (AS1- Streptomyces lucensis, AS2-Streptomyces griseus and AS3- Streptomyces olivaceus) the antagonistic assay was done using dual culture method in nine combinations of actinomycetes and rhizobium isolates. Out of nine combinations of antagonistic assay, eight combinations showed that Actinomycetes executes an inhibitory effect on growth of rhizobium strain, only one combination (AS3 against RS3) showed a reverse effect where rhizobium isolates (RS3) had higher zone of growth than actinomycetes isolate (AS3) The antagonistic action of streptomyces griseus (82%) and streptomyces olivaceus (71%) were maximum against rhizobium pusense, 18% and Sinorhizobium meliloti 29% respectively while other species showed varied but lower activity than these two actinomycetes species.

Keywords: Antagonistic assay, isolates, actinomycetes, rhizobium, dual culture, field pea.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More