Abstract

ESTIMATION OF LUPEOL IN ACHYRANTHES BIDENTATA BLUME. BY HPTLC METHOD

Raghbir Chand Gupta, Nusrat Fayaz and Saroj Kumar Pradhan*

ABSTRACT

A rapid and simple high performance thin layer chromatographic method was established for the identification and the quantification of lupeol in Achyranthes bidentata. The separation of the compound was done on the pre coated silica gel 60F254 and solvents n-hexane: ethyl acetate (8:2 v/v) as for the elution of marker compound from the plant extracts. The calibration curve was found to be linear between 2- 10μg/band with correlation coefficient of 0.996. The amount of lupeol in 50% methanolic extracts of leaf and stem samples was found 12.35 and 8.30mg/gm DWE respectively. The developed technique is simple, accurate, rapid and selective for the estimation of lupeol from Achyranthes bidentata and other herbal formulations.

Keywords: A. bidentata; HPTLC; Lupeol.