Abstract

ESTIMATION OF ANALYTICAL PARAMETERS OF TLC-METHOD FOR ANALYSIS OF DOCOSAHEXAENOIC ACID AS COMPOUND OF OMEGA-3 POLYUNSATURATED FATTY ACIDS

Bozhidarka Radoslavova Hadzhieva, Dobrina Doncheva Tsvetkova*, Danka Petrova Obreshkova

ABSTRACT

The aim of current study was the estimation of the analytical parameters linearity, accuracy and precision of simple TLC-method for analysis of Docosahexaenoic acid, as a component of Omega-3 polyunsaturated fatty acids. The aim was to be proposed simple, easy and not expensive method. After the experiments with different mobile phases, was proved that the most appropriate mobile phase was: cyclohexane : ether : benzene = 8 : 4 : 1 v/v/v. The developed Silicagel G60F254 plates were dried on air and the plates were examined on UV-lamp at λ = 254 nm. The selectivity of the applied TLC-method was confirmed by the fact, that on chromatogram with placebo preparation did not exist spot with Rf, corresponding to RfDocosahexaenoic acid = 0.3. The linearity accordance between the concentration and spot area in concentration range: 2.10–3 g/ml ÷ 5.10–2 g/ml was proved by the regression equation: y = 44.11.x + 0.038.

Keywords: TLC, Docosahexaenoic acid, Omega-3 fatty acids.