Abstract

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR DETERMINATION OF CIPROFLOXACIN AND MALONDIALDEHYDE IN PLASMA.

Monali Wawre*, Sukeshini Lote and Agrasen Moon

ABSTRACT

This study establishes the applicability of using high-performance liquid chromatography with UV- Visible detection for the simultaneous determination of malondialdehyde (MDA) and Ciprofloxacin (Cipro). HPLC pump Spectra System P4000 with Prontosil C18 {length 250mm× 4.63 (I.D), 5μm particle size.} column and UV-1000 with Data Ace Software detector was used for the study. The standard and sample solution of Ciprofloxacin was prepared in mobile phase. MDA in vitro was derivatized with TBA to form MDA-TBA complex, the sample solution was prepared in mobile phase. During selection and optimization of the mobile phase it was observed that the sharpness and resolution of the peak is achieved with decreasing the proportion of acetonitrile .The mobile phase that was found to be most suitable was water with 0.2% Triethylamine, pH adjusted to 2.3 by ortho-phosphoric acid: ACN in ratio 80:20 v/v, flow rate 1ml and UV detection at 278 nm for both Cipro and MDA-TBA complex. The retention times of MDA-TBA and Cipro were 3.3 and 6.5 min, respectively. To the best of our knowledge, this paper describes the first practical analytical approach toward simultaneously monitoring the levels of MDA and Cipro in plasma. The Cipro-induced oxidative stress measured using this method indicated that CIPRO treatment did markedly increase the level of MDA. Theme of the work was to study the effect of Ciprofloxacin (Fluroquinolones Antibiotic) on level of oxidative stress Biomarker Malondialdehyde.

Keywords: Bioanalytical method, Malondialdehyde, Ciprofloxacin, Oxidative Stress.