Abstract

NEPHROPROTECTIVE EFFECT OF ETHANOLIC EXTRACT OF FLOWERS OF MICHELIA CHAMPACA AGAINST CISPLATIN-INDUCED

Satyavati. D*, Shyamalendu Tripathy, Srinivas. K

ABSTRACT

The present study was designed to evaluate the Nephroprotective effect of Michelia Champacaflowers ethanolic extract in Cisplatin-induced nephrotoxicity. Wistar rats were divided randomly into four groups of six animals each (n=6) receiving different treatments, consisting of vehicle (normal saline 2ml/kg b.w), single dose of cisplatin (5mg/kg of body weight; i.p), standard polyherbal drug cystone at a dose of 5ml/kg body weight,Michelia Champaca flowers ethanolic extract (250mg/kg body weight) for 14 days. Nephroprotective activity was assessed by estimating various biochemical parameters likeserumurea, creatinine, Blood urea nitrogen (BUN), uric acid, total protein and haematological estimations like Hemoglobin (Hb), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), Total leukocyte count (TLC) and Red blood cells count (RBC).Histological analysis of rat kidneys and body weights were also observed. Measurement of antioxidant parameters- lipid peroxidation, catalase, Superoxide dismutase and reduced glutathione was also investigated. Administration of Michelia Champacaflowers ethanolic extract produced significant nephroprotective activity in cisplatin induced nephrotoxicity model as evident by decrease in elevated serum creatinine, urea, BUN, uric acid and total protein levels, which was further confirmed by histopathological study. The nephroprotective activity of Michelia Champacacould be due to the inherent antioxidant and free-radical-scavenging principle(s) contained in the extract.

Keywords: Michelia champaca, cisplatin, Nephrotoxicity and antioxidant activity.