Abstract

EXTRACTION, ISOLATION AND ANTIBACTERIAL EVALUATION OF CRUDE AND PURIFIED FERRITIN EXTRACT FROM SEEDS OF CICER ARIETINUM L.

Supriya Kapil Shetty Thanekar*, Ramachandra Y L, Udgire M

ABSTRACT

The present study aimed to isolate, purify and antibacterial evaluation of ferritin extracted from Chick pea (Cicer arietinum L.) against Gramnegative microorganisms (Escherichia coli, Pseudomonas aeruginosa, Kliebsiella Pneumonia, Proteus vulgaris) , as well as Gram-positive microorganism (Staphylococcus aureus, Staphylococcus epidermis). Agar well diffusion method was adapted to determine antibacterial evaluation against all the test microorganisms. Zone of inhibition of the crude and pure extracts were tested. Among all the test pathogens E. coli was found susceptible with zone of inhibition 8 mm. to the purified ferritin extract. Cicer arietinum L. ferritin was successfully isolated with two subunits with molecular weights of 20.1- kDa and 29- kDa respectively .The present study reveals the least susceptibility of the microorganisms towards the ferritin isolated from seeds of Cicer arietinum L.

Keywords: Ferritin, Purification, Cicer arietinum L., Antibacterial evaluation.