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Abstract

A NEW RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF QUINAPRIL AND HYDROCHLOROTHIAZIDE IN PHARMACEUTICAL DOSAGE FORMS

G.Sujita Rani, Mohan Gandhi.B*, G.Mani Kumar,M.Rekha, K.Pullam Raju ,N.Manjusha

ABSTRACT

A novel, precise, accurate, rapid and cost effective isocratic reversephase high performance liquid chromatographic (RP-HPLC) method was developed, optimized and validated for the estimation of Quinapril (QPL) and Hydrochlorothiazide (HTZ) in pharmaceutical dosage forms (tablet). The drugs were estimated using Hypersil BDS C18 (150 mm x 4.6 mm i.d- 5 μm particle size) column. A mobile phase composed of tri ethylamine buffer, acetonitrile in proportion of 60:40 v/v, at a flow rate of 1.0 ml/min was used for the separation. Detection was carried out at 220 nm. The linearity range obtained was 50-150 μg/ml for Quinapril and Hydrochlorothiazide with retention times (Rt) of 4.803 min and 2.713 min for Quinapril and Hydrochlorothiazide respectively. The correlation coefficient values were found to be 0.997 & 0.998. Precession studies showed % RSD values less than 2 % for both the drugs in all the selected concentrations. The percentage recoveries of QPL and HTZ were in the range of 99.70-101.50 % and 98.80-100.40 %. The assay results of QPL and HTZ were 100.70 % and 99.30 % respectively. The limit of detection (LOD) and limit of quantification (LOQ) were 0.0592 μg/ml and 0.1793 μg/ml for QPL and 0.0509 μg/ml 0.1543 μg/ml for HTZ respectively. The method was validated as per the International Conference on Harmonization (ICH) guidelines. The proposed validated method was successfully used for the quantitative analysis of commercially available dosage form.

Keywords: Quinapril, Hydrochlorothiazide, RP- HPLC, Hypersil BDS C18 Column, Validation.


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