Abstract

CLONING, SEQENCING AND BIOINFORMATIC ANALYSIS OF BANANA STREAK VIRUS (BSV)ISOLATES

Dr. K. Anandhi* and M.Vijayalakshmi

ABSTRACT

Banana streak disease caused by Banana streak virus (BSV) is one of the serious viral diseases of banana in India. Streak virus infected banana leaf samples were collected from different geographical regions. The sample was initially tested by Direct Antigen Coating Enzyme Linked Immunosorbent Assay (DAC-ELISA) using recombinant polyclonal antiserum viral associated protein (BSMYV-rVAP). The partial RT-RNaseH region of the viral genome was amplified from all samples and all those samples yielded approx. 600 bp product further confirmed the existence of virus in the samples. The Partial polyprotein gene of BSV isolate shared identity of 99.4-99.5% at nucleotide level (nt) and 99.3-99.7% at amino acid (aa) level with BSMYV-AUS isolates. This isolate shared 48.6-58.4 per cent sequence homology at nucleotide level and 42.4-52.8% at amino acid level with all other BSV species.

Keywords: ? DAC-ELIZA- direct antigen coating enzyme linked immunosorbent assay, ? BSV- Banana Streak Virus ? Phylogenetic analysis