Abstract

STANDARDIZATION AND ANALYTICAL EVALUATION OF TRADITIONAL SIDDHA FORMULATION THIRISOOTHA MEZHUGU: A MODERN ANALYTICAL APPROACH.

A. M. Dharsana*, P. Shanmuga Priya, C. Parkavi, J. Kanimozhi, S. Vanathi

ABSTRACT

Siddha system of medicine has distinguished itself from the rest of the traditional medicines by its compassionate way of understanding the human disease and also by the unique way of treating the dreadful disease. As the practice of siddha system of medicine hikes day by day and now people around the globe started realizing the importance of siddha formulations and its applications towards various diseases. Though Siddha system has its own value the concern on availability of physicochemical and standardization data of most of the siddha formulation is high doubtful. Hence standardization of siddha drugs becomes highly essential in order to explore its potency and efficacy in the global market. As a consequence of focusing upon the current need of drug standardization the present work undertaken to standardize the traditional herbomineral siddha formulation Thirisootha mezhugu (TM) which has been used for the treatment of various ailment like hemiplegia and lumbar spondylosis. Still now there is no proper documentary evidence available on standardization aspect of this formulation this prompted us to peruse the systematic standardization of TM by qualitative, phytochemical and physiochemical evaluation by AYUSH guidelines, particle size distribution by Scanning Electron Microscope (SEM) and heavy metal by inductively coupled plasma optical emission spectrometry (ICP-OES) analysis. The results obtained from physiochemical analysis clearly reveals that total ash value of TM is about 19.63% in which acid insoluble ash is 0.62% and water soluble ash is about 1.42%. Extraction value of drugs renders most significant information about partition of the active component the results of extract value study shows that alcohol soluble extract of TM is 31.80% and water soluble extract is 47.70%. Result obtained from the phytochemical analysis reveals the present of alkaloid and starch, further result of qualitative chemical analysis shows the presence of silicate, carbonate and chlorides. The results obtained from the SEM analysis shows that most of the particles present in TM are in micro size ranges from 1μm – 2 μm. The results of the present investigation render some valuable information about the siddha formulation Thirisootha mezhugu to the future researchers and also provide evidence based information on standardization part of this noble formulation.

Keywords: Standardization, Siddha system, Thirisootha mezhugu, SEM, ICP-OES, Phytochemical, Physiochemical analysis.