Abstract

EFFECT OF NIGELLA SATIVA L. ON LIVER ENZYMES AND KIDNEY MORPHOLOGY ON D-GALN INDUCED TOXICITY IN RATS

Dr. A. Mohamed Saleem Gani* and Dr. S. Ahmed John

ABSTRACT

The present work has been carried out to study the effect of alcoholic extract of Nigella sativa L. seeds (NS) on liver enzymes and kidney cells in rats intoxicated with D-Galactosamine(D-GalN). Twenty four adult wistar albino rats were divided into four groups viz Control, DGalatosamine induced hepatotoxicity, Nigella sativa alcoholic extract (NSE) treated and Nigella Sativa alcoholic extract pretreatment prior to the administration of D-Galactosamine. After treatment, the rats were sacrificed, blood samples were collected for determination of Serum Aspartate transaminase (AST), Alanine trasnaminase (ALT) and Alkaline phosphatase (ALP) and the kidneys were isolated for histopathological study. Mean AST, ALT and ALP levels were found to be significantly higher in D-GalN induced hepatotoxic rats group (P<0.05) than in the control group. Kidney cells of D-GalN administered rats group shows changes in glomeruli, lesions in nephron structures and tubular degeneration. Levels of these parameters in the NSE pretreated group were found to be nearer to control and the kidney cells of NSE pretreated group did not show any adverse histological pattern. Results of the present study strongly indicate that NS having hepatoprotective efficiency againt D-GalN induced toxicity in rats.

Keywords: Nigella sativa L., D-Galactosamine, Liver enzymes, Kidney, Histopathology & Rats.