Abstract

IN VITRO ANTIOXIDANT ACTIVITY, PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY OF VARIOUS TRIPHALA EXTRACTS.

Bilal Ahmad Dar*, Shah Khalid, Ajaz Ahmad Wani, Sheeraz Ah Teli, Amar Sohail Mirza

ABSTRACT

Aim: To Investigate In vitro antioxidant activity, Phytochemical analysis and Antimicrobial Activity of Triphala an Indian medicinal preparation (IMP). Methods: 1. Phytochemical investigations of Triphala Chloroform, Petroleum Ether and Methanol extracts were done with standard methods. 2. In vitro antioxidant activity of methanolic extract of Triphala was determined by DPPH assay. 3. Antimicrobial activity of Various Triphala Extracts was determined by Disk diffusion method. Result: The Phytochemical investigation of different extracts showed presence of all most all of the phytochemicals except proteins and flavonoids. Methanolic extract was showed to possess significant antioxidant property. Furthermore, the methanolic extract showed potential antimicrobial activity as compared to other extracts. Conclusion: Triphala possess excellent antioxidant activity and thus can be used as potent agent against free radicals. The extracts showed huge antimicrobial activity. Therefore, it acts antagonistic to various Gram +ve and Gram –ve bacteria and thus it is very important drug from the pharmaceutical and medicinal science point of view.

Keywords: Triphala Extract, Phytochemicals, In vitro, Biological properties.