Abstract

EVALUATION OF ANTIOXIDANT POTENTIAL, PHENOL AND FLAVONOID CONTENTS OF DIFFERENT EXTRACTS FROM THE LEAVES OF Pleiospermum alatum (WALL. & ARN.) SWINGLE

D. Umaiyambigai, K. Saravanakumar and G. Adaikala Raj*

ABSTRACT

Antioxidants are the substances that may protect cells from the damage caused by free radicals. Antioxidants interact with and stabilize free radicals and may prevent some of the damage free radicals might otherwise cause clinical disorder. The aim of the present study was to determine the in vitro antioxidant property of petroleum ether, chloroform, ethyl acetate and methanol extracts of Pleiospermum alatum (Wall. & Arn.) Swingle. The different extracts of P. alatum leaves were antioxidant potential by using 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2, 2-Azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS•+ ), Hydrogen peroxide scavenging, Superoxide anions scavenging, Hydroxyl radical scavenging, Ferric reducing antioxidant power, Total antioxidant activity (Phosphomolybdic acid) IC50 values were calculated compare to standard antioxidant for L- ascorbic acid and (BHT) butylated hydroxytoluene, gallic acid. The methanol extract showed the highest total phenol and flavonoid contents were also investigated. Among the different extracts methanol extract of P. alatum had significantly higher. The total phenol (6.38 ±0.76) mg/ml gallic acid equivalent (GAE/g) and flavonoids (8.18 ± 0.37) mg/ml quercetin equivalent (QE/g) were found to be higher in methanol extract of P. alatum. The results of the study revealed that the P. alatum can be an interesting source of natural antioxidants with their potential use in different fields namely food, cosmetics, medical and pharmaceuticals.

Keywords: Pleiospermum alatum, Free radical scavenging activity, DPPH, ABTS•+, FRAP.