Abstract

TO STUDY THE EFFECT OF SILYMARIN AGAINST LIPOPOLYSACHHARIDES INDUCED NEUROINFLAMMATION IN MICE

Priyanka Sonker*, Talha Jawaid, Polly Gupta, Kalpana and Rajan Awasthi

ABSTRACT

Aim of the study: To study the effect of Silymarin against Intracerebral (i.c.) LPS induced Neuroinflammation in mice. Material and methods: The effect of Silymarin was examined against Intracerebral (i.c.) LPS induced Neuroinflammation in mice. The behavioural study was performed by using models Morris water maze. Various biochemical parameters such as proinflammatory cytokines (TNF-α) as indicator of Neuroinflammation, Acetyl cholinesterase (Ach E) activity as marker of cholinergic activity and malonaldialdehyde ( MDA) and glutathione (GSH) as marker of oxidative stress were estimated. LPS was used in dose of (5μg/5μl) for Neuroinflammation in mice was used. Silymarin was administered in two different doses of 25mg/kg and 50mg/kg. Result: Silymarin exhibit a significant (p<0.05) decrease in latency time (LT) in Morris water maze model indicating memory enhancing activity of the drug in dose dependent manner. Also, (TNF-α) were significantly (p<0.05) reduced in mice brain as well as brain indicating antineuroinflammatory activity of drug. MDA level and Ach E activity was reduced significantly (p<0.05) in treated group in comparison to LPS treated group indicating anti oxidant effect of Silymarin. Conclusion: In conclusion, the present study indicated that Silymarin could ameliorate memory impairment induced by lipopolysachharide. Intracerebral microinjection of LPS ((5μg/5μl) significantly induced Neuroinflammation in mice and cause release of cytokines (TNF-α), free radical generation and oxidative stress in the brain regions. Treatment with Silymarin in two different doses (25mg/kg and 50 mg/kg) effectively attenuated LPS induced Neuroinflammation, oxidative stress and cognitive impairment.

Keywords: Silymarin, LPS, MDA, GSH, Ach E, Intracerebral (i.c.) and Latency time (LT).