Abstract

EVALUATION OF GENOTOXIC POTENTIAL OF BLOXIVERZ (NEOSTIGMINE METHYLSULFATE) ON MEIOTIC CELLS OF MOUSE

Dr. D. Rathna Prabha* and Dr. M. Ganga Raju

ABSTRACT

Neostigmine is an anti muscle relaxant used in post operation care to relieve the patient from anaesthetic effects. Today it is the most common agent used for the reversal effects of other agents used for neuromuscular blocks, like Suxamethonium chloride, Atracurium etc. In our study, in vivo experiments were conducted on meiotic tissue (Testes) of Swiss albino mice to assess the genotoxic effects of the drug on meiotic chromosomes and sperms. Human therapeutic doses were converted to mice body weight basis ranging from minimum to maximum (2, 8 &20μg.) Experiments were conducted in single and cumulative dose series. Slides were prepared from testes and cauda epididymis of control and treated mice after 24hr., Ist, IInd, IIIrd, IVth and Vth weeks by following standard air drying technique and stained with 2% Giemsa. The drug did not induce chromosome aberrations and point mutations for all periods and doses to a significant extent in both series. The observations indicate that Neostigmine is not genotoxic on meiotic cells of mouse.

Keywords: Bloxiverz, Neostigmine, Genotoxicity, Chromosome aberrations, Sperm Head Abnormalities, point mutations.