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Abstract

ANTIBACTERIAL ACTIVITY OF NANOPARTICLES SYNTHESIZED FROM CORIANDRUM SATIVUM LINN FRUIT

M Padmaa Paarakh* and Kansara Swati

ABSTRACT

The antibacterial activity of methanol extract and nanoparticle synthesized using methanol extract of fruit of Coriandrum sativum Linn were tested against Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris, Escherichia coli and Klebsella spp. The in vitro antibacterial activity was performed by agar well diffusion method. The zone of inhibition in mm was compared with the standard drug i.e. ciprofloxacin. The zone of inhibition for methanol extract were found to be 11 mm,10 mm,7 mm,none and 9 mm for Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris, Escherichia coli and Klebsella spp respectively. The zone of inhibition for Silver nanoparticles were found to be 12 mm,12 mm,12 mm,11 mm and 12 mm for Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris, Escherichia coli and Klebsella spp respectively. The zone of inhibition for Zinc nanoparticles were found to be 11 mm,8 mm,11 mm, none and 7 mm for Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris, Escherichia coli and Klebsella spp respectively. The zone of inhibition for magnesium nanoparticles were found to be 7 mm,10 mm,8 mm, 8 mm and 8 mm for Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris, Escherichia coli and Klebsella spp respectively. The minimum inhibitory concentration[MIC] for Bacillus subtilis was 2 mg, 100,250 and 50 μg/ml; MIC for Staphylococcus aureus was 2 mg,100,100 and 250 μg/ml; MIC for Proteus vulgaris was 2 mg, 100, 500 and 100 μg/ml; MIC for E.coli was none, 100, none and 100 μg/ml and MIC for Klebsella spp was 2 mg,50, 500 and 100 μg/ml for methanol extract, silver nanoparticle, zinc nanoparticle and magnesium nanoparticle respectively suggesting the antibacterial activity of Coriandrum sativum and nanoparticle synthesized.

Keywords: Coriandrum sativum, silver nanoaprticles, zinc nanoparticle, magnesium nanoparticle, antibacterial activity, zone of inhibition, minimum inhibitory concentration.


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