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Abstract

OPTIMIZATION OF NUTRIENTS FOR L-ASPARAGINASE PRODUCTION USING ASPERGILLUSTERREUS

M Anamika, Y Neeraja, J Swarnalatha, S Venkateshwar, L Venkateswar Rao*

ABSTRACT

The objective of this work was to produce L-asparaginase from Aspergillus terreus. L-asparaginase was produced through submerged fermentation by using modified czapekdox medium. In this study the activity of asparaginase determined by different carbon and nitrogen sources.The modified Czepak-Dox media with 0.5% Sucrose, 1% asparagine, Zn+2 metal ion, triton-X surfactantsshowedmaximumLasparaginase activity of 34.25 IU/mL on the third day.

Keywords: Aspergillus terreus, modified czapekdox medium, L Lasparagine,


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