Abstract

DETERMINATION OF 6-GINGEROL IN GINGER VARIETIES OF INDIAN SUBCONTINENT BY RP-HPLC METHOD

Kamran Ashraf*, and Mohd Mujeeb

ABSTRACT

A rapid and sensitive High Performance Liquid chromatography method was developed and validated for the determination of 6-gingerol in the different rhizomes of Zingiber officinale Rosc. on a C18 reverse phase column (25 x 4.6mm, particle size 5 μm, Merck, Germany) column using isocratic mode acetonitrile: water (90:10 v/v) at a flow rate of 1 mL/min. The analysis time was 10.0 min and the elution of 6- gingerol occurred at 4.20 min. The linear dynamic range was established over the concentration range of 1 and 500 μg/mL for the analyte. The limits of Detection and Quantitation for 6-gingerol was found to be 0.32μg/mL and be 1.02μg/mL respectively. The relative standard deviation and accuracy were found to be satisfactory. The results showed significant variation occurred (0.176%–0.290% % w/w) in gingerols among twelve accessions and the Patna variety contained the highest concentrations of 6-gingerol and was concluded to be superior. These results may be used to devise strategies for cultivating this plant for large scale production.

Keywords: HPLC, Ginger, Validation.