Abstract

NANOPARTICLES PLAY MULTIPLE ROLES IN THE TARGETED DRUG DELIVERY SYSTEM IN THE TREATMENT OF PROSTATE CANCER IN MODERN TRENDS

Nouratan Singh* Prof. Dr. Dalel Singh, Dr. Neeraj Tandan, Prof. Dr. S.P. Singh,
Surya Pratap Singh and Anil Yadav

ABSTRACT

The common occurrence is shrinkage of cancer after first line therapy with anticancer agents. This recurrence is mainly believed that due to the presence of cancer stem cells in the tumor. Therefore, a combination therapy, susceptible to both types of cells is desirable to reduce the size of tumor. Delivery of this combinatorial therapy approach in a drug delivery system will provide even a better outcome in therapeutic for treatment and tumor targeting. The main objectives of this study was to characterization and development of nanoparticles for drug delivery system containing two anticancer agents first is cyclopamine and the second paclitaxel having different susceptibilities against cancer cells. Both drugs were enticed in chitosan and glyceryl monooleate (C-GMO) solid lipid as well as poly lactic-co-glycolic acid (PLGA) nanoparticles. The particle size of drug-loaded C-GMO nanoparticles was 278 ±16 nm with a positive zeta potential and the PLGA particles size were 234±06 nm with negative zeta potential. The analyses of both nanoparticles acknowledge that the drugs were present in non-crystalline state in the matrix. PLGA blank particles showed no cytotoxicity in all the tested cell lines, whereas C-GMO blank particles showed massive cytotoxicity. The quality of polymer used for the preparation of nanoparticles play a major role and affected the in vitro release, create cytotoxicity and uptake of nanoparticles in the all the tested cell lines.

Keywords: Cytotoxicity, cancer stem cells, prostate cancer, Nanoparticles, combination therapy.