Abstract

EVALUATION OF PHYTOCHEMICALS ACCOUNTABLE FOR ANTIMICROBIAL ACTIVITY OF ANDROGRAPHIS PANICULATA AND TINOSPORA CORDIFOLI EXTRACTS.

Kinnari N. Mistry*, Bhoomi B. Joshi and Chaitali Y. Patel

ABSTRACT

Aim: A huge group of natural antimicrobial compounds are active against a large spectrum of bacterial strains causing infectious threat. The present study was aimed to evaluate the phytochemical playing vital role in antimicrobial activity of different crude extracts of Andrographis paniculata (leaf powder) and Tinospora cordifolia (stem powder). The fresh part of plant (leaves) of A.paniculata and (stems) of T.cordifolia were collected from Boriavi, Anand. Crude extract of both these plants were further screened for active phytochemicals that are responsible for anti-microbial activity. The anti-microbial activity of these plants was further checked and compares by calculating their MIC values along with standard drug. The accuracy of the information was then compared against published data. These plants extracts gave higher total % yield in hot condition compared to cold condition. Phytochemical screening confirmed the presence of alkaloids, terpanoids, flavonoids, phenols and free anthraquinones. Ethanolic extract of both the strains showed good antimicrobial activity carried out by agar well diffusion method against six different strains Andrographis paniculata showed maximum effect was at dose of 7 mg/ml where as Tinospora cordifolia maximum effect was observed at dose of 40 mg/ml. In both the plants ethanol extract exhibited maximum zone of inhibition with different concentration using streptomycin as positive controls. The present study indicates that there is a need to search for usage and pharmacological activities of bioactive compounds isolated from Indian medicinal plant having antimicrobial properties.

Keywords: Andrographis paniculata, Tinospora cordifolia, Phytochemicals, Antimicrobial activity.