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*Dhanashri B. Nagulwar1, Pravin K. Bhoyar2, Jagdish R. Baheti3, Dinesh M. Biyani5, Dharmendra R. Mundhada4 , Prasad P. Kathade2

1Department of Pharmaceutics, Sharad Pawar College of Pharmacy, Dist: Nagpur, Maharashtra, India.
2Siddhivinayak College of Pharmacy,Warora, Dist-Chandrapur, Maharashtra, India.
3Shriman Suresh Dada Jain College of Pharmacy, Chandwad, Nasik, Maharashtra, India
4Department of Pharmaceutics, Agnihotri College of Pharmacy, Wardha, Maharashtra, India
5Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Dist- Ranchi, Jharkhand, India.


For skin infections, topical treatments applied directly to the skin are safest. Present work was decided to carry out antimicrobial activity and antiseptic effect of formulated cream. Oil of J. regia oil of K. galanga, methanolic extract of C. tora seeds and T. arjuna bark were successfully extracted and phytochemically screened. Thin layer chromatography of above extracts showed that it contains different active constituents. The result of phytochemical screening reveals that the major constituents of extracts were coumarins, flavonoids, steroids and triterpenoids. The in- vitro antimicrobial activity showed that the oil, methanolic extract and formulated cream posses prominent activity compared with that of standard. Zone of inhibition were found to be prominent in concentration 5μg / ml of oil and 155ug / ml for C. tora seeds extract. In antimicrobial activity of cream, the formulation containing maximum percent of oils i.e. 5% and 155ug/ml of C. Tora methanolic extract showed highest zone of inhibition. Validation of cream was done and was found within the limits. The pH of cream was found to be in range of 6.5 7.0 which is good for skin pH. The consistency of cream was in the range of 230 to 290 1/10mm. The formulated cream was used for antiseptic action. Patient trial was carried out and successful results were seen. For this purpose cream was applied topically. In human study, trials were conducted on 10 patients suffering from burn infection, tinea pedis and acne. Signs of the infection were healed in the period of 2 week.

Keywords: Skin, C. tora, tinea pedis, acne.

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