Abstract

THE NOVEL USE OF MULTIWALLED CARBON NANOTUBES-BASED SENSORS FOR VOLTAMMETRIC DETERMINATION OF ITRACONAZOLE: APPLICATION TO PHARMACEUTICAL DOSAGE FORM AND BIOLOGICAL SAMPLES THROUGH SPIKED URINE SAMPLE

Maha A. Sultana , Ali K. Attiab , Maha M. Abou El-Alamina, Mostafa A. Atiaa*

ABSTRACT

Simple, sensitive and precise voltammetric method was developed and validated for determination of commonly used antifungal itraconazole (ITR) in bulk powder and tablet dosage form, as well as in spiked human urine. The electrochemical activity of ITR was investigated at the surface of multi-walled carbon nano tubes modified carbon paste electrode (MWCNTs/CPE). The cyclic voltammogram (CV) showed a prominent irreversible anodic peak (oxidation) at potential of 745 mV in 0.04 M Britton Robinson buffer (pH 5.0): Methanol (1:1 v/v). Various experimental conditions were investigated and optimized, such as different types of surface modifiers on MWCNTs/CPE, type of supporting electrolyte, pH, accumulation time and scan rate. A good linear relationship was obtained between the concentration and the height of the peak within the concentration range 1.55-44.68 μg/ml (R2 = 0.9999) with limit of detection and quantification of 0.51 μg/ml and 1.55μg/ml respectively. The intra-day and inter-day relative standard deviation were ≤ 1.3 and 2.03, respectively. The results obtained were fully validated according to the International Conference on Harmonization (ICH) guidelines and found to be in accordance with those obtained by the official method. The proposed method was successfully applied for the determination of ITR in its commercial dosage form and spiked human urine with percent recovery 100.23±0.35 and 99.70±1.60 respectively.

Keywords: MWCNTs/CPE, cyclic voltammogram (CV), ICH.