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Dr. Narayanaswamy S. Y.* and M. Ramachandra Mohan


The neem oil is a useful local remedy in some chronic forms of skin diseases and ulcers. It is also antiseptic and commonly used for animals both internally and externally. The oil is also extremely useful as a parasiticide. Fish is a nutritious food for peoples, hence a gonad of Glossogobius giuris observed during spawning phase on treatment with sub lethal concentrations of Neem oil (0.05 ppm, 0.25 ppm and 0.5 ppm) for 24, 48, 72 and 96 hrs intervals. The large and sexually mature female fishes were used in this study. The body length and weight of fishes were recorded. The female fish weighing about 20-50 g and length 110 to 220 mm were selected for ovaries, the ovaries contain ripen eggs with large oocytes, well defined nuclei, distinct nucleoli and a few immature oocytes. The results of the present study showed morphological changes in the cell structure, the ovarian growth was inhibited, the oocytes and interstitial tissues were encapsulated by a connective tissue, capsule consisting of germinal epithelium and tunica albuginea with blood vessels. As the concentration increases, the amount of atretic follicles increased and the ovarian wall became more thickened and lifted. The ovaries of fish exposed to higher concentration an immense amount of empty cells along with necrosis of nuclei have seen.

Keywords: Neem oil, G. giuris, oocytes, nucleoli and tunica albuginea.

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