Abstract

DNA EXTRACTION OPTIMIZATION IN SORGHUM [SORGHUM BICOLOR L. MOENCH] FOR MARKER ANALYSIS

Anita Grewal*, Archit Sharma, Surender Pahuja and Sunita Khatak

ABSTRACT

Studies were undertaken for optimization of DNA extraction from Sorghum for marker analysis, fifteen different type of Sorghum genotypes (have different characters) were selected for this study. For any maker analysis, there is need of purified DNA sample which is extracted in simple as well efficient manner. So, this is the modified method of CTAB procedure which involves high concentration of NaCl in clean up buffer and CTAB (2%), β-mercaptoethanol (0.2%), EDTA (0.5M) in extraction buffer to remove polypenols and polysaccharides and then use of potassium acetate and cholorform to remove protein and polysaccharides. After that DNA was precipitated in isopropanol, finally DNA was purified by giving RNase treatment and quality of DNA was determined by agarose gel electrophoresis.

Keywords: DNA extraction, Sorghum, polyphenols, polysaccharides,