Abstract

IN VITRO DNA PROTECTIVE POTENTIALS OF HYDROETHANOLIC EXTRACTS OF SOME MEDICINALY IMPORTANT INDIAN FLOWERS.

Nitin Puranik*, Sarika Jain, Santosh Gaherwal, Sonica Ojha, Shilpa Kushwaha

ABSTRACT

Hydroethanolic extracts of Butea monosperma, Nerium oleander, Mesua ferrea, Nelumbo nucifera, and Calotropis gigantea flower were prepared and assessed for their DNA damage Protective effect in vitro using isolated goat liver DNA as model system and the efficacy of protective potentials was compared to Ascorbic acid as standard antioxidant. Fenton reagent used as damaging agent which generates free radicals in system and thus causes oxidative DNA damage. In order to protect DNA we incubated the DNA with the ethanolic extracts of Butea monosperma, Nerium oliender, Musea ferra, Nelumbo nucifera, and Calotropis gigantea flower. All the flower extract showed good protective potentials to inhibit oxidative damage in DNA and thus retains the entity of treated DNA.

Keywords: Hydroethanolic flower extract, Butea monosperma, Nerium oliender, Musea ferra, Nelumbo nucifera, Calotropis gigantea, oxidative DNA damage, Fenton reagent.