Abstract

ACTIVATION OF CEPHALOPODS INK ON MACROPHAGES AGAINST RAW 264.7 CELL LINE

Jemma Hermelin Jesy Diaz* and R. D. Thilaga

ABSTRACT

Effect of crude and fractionated protein ink of L.duvauceli (squid) and S.pharaonis (cuttlefish) were accessed using macrophage scavenging assay against RAW 269.7 cell line in four different concentrations(10,100,500 and 1000μg/ml). The crude ink of L.duvauceli at 10μg/ml concentration showed the activity of 8% and increased to 75% at 1000μg/ml of concentration. Fractionated protein squid ink (30%) showed minimum (12%) scavenging activity at 10μg/ml concentration and maximum of 82% at 1000μg/ml concentration. The macrophage scavenging activity has increased as the concentration of ink was increased. Scavenging activity of crude ink of S.pharaonis ranged from 3% (10μg/ml) to 60% (1000μg/ml). Whereas the activity of 70% was observed at 1000μg/ml concentration against 80% fractionated protein cuttlefish ink. The percentage of activity was higher in the fractionated protein ink of S.pharaonis when compared to crude protein. In the present study fractionated protein ink from squid and cuttle fish showed better macrophage scavenging activity compared to crude ink. Increase in activity indicates the importance and better efficiency of samples.

Keywords: Crude ink, fractionated protein ink, S.pharaonis, L.duvauceli and macrophage scavenging activity.