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Abstract

STANDARDIZATION OF RUBIA CORDIFOLIA AND ITS FORMULATIONS USING ALIZARIN AS MARKER COMPOUND BY HPTLC

Archana M. Gorle*, Suvarna P. Phadatare, Rupesh Pingale,
Kalpana Tilekar, Deepak Pokharkar and Dr. Swati Patil

ABSTRACT

A simple and reproducible HPTLC method for the determination of Alizarin in Rubia cordifolia and its formulations was developed and is described. The HPTLC method involve separation of components by TLC on stationary phase i.e. precoated silica gel GF60254 (20x10 cm, aluminium sheet) with a solvent system of toluene: ethyl acetate: formic acid (9.8:0.2:0.1) and detection was carried out by scanning and quantifying the peak at 254 nm for Alizarin . The sensitivity of HPTLC method was found to be 50 ng and the linearity was observed in the range of 0.1 g to 1g. The method was validated in terms of linearity and specificity. The proposed method being precise and sensitive can be used for detection, monitoring and quantification of Alizarin in Rubia cordifolia and its formulations.

Keywords: Rubia cordifolia, Formulations, HPTLC, Alizarin, Standardization.


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