Abstract

IN VITRO CYTOTOXIC ACTIVITY OF VARIOUS FRACTIONS OF HYDROALCOHOLIC EXTRACT OF VITEX PINNATA LINN LEAVES AGAINST EAC CELL LINES

S. Thenmozhi* and U. Subasini

ABSTRACT

Vitex pinnata Linn. is a moderately sized tree of tropical Asia belonging to the family Verbenaceae. Vitex pinnata is the tree with trifoliate leaves, gray to brown bark. The leaves are potential as antioxidant; because of flavonoids, alkaloids & terpenoids content. In Traditional medicine, the plant is used to expel intestinal worms, as analgesic, anti-inflammatory, antipyretic, wound healing, anti-oxidant, antibacterial and stomach ache. The present study deals with the in vitro anticancer activity of various fractions of hydroalcoholic extract of Vitex pinnata Linn. against L6 and EAC cell lines. MTT assay was used to assess the in vitro anticancer activity. The extract at various doses were treated against all the cell lines. The parameters analyzed were percentage of cytotoxicity, percentage of cell viability and IC50 values were evaluated. In results the ethanolic fraction of Vitex pinnata showed more potent activity than the all other fractions. All the fractions did not show any cytotoxicity against the normal L6 cell line. Among the five fractions, the petroleum ether & chloroform fractions showed minimal activity against EAC cell lines compared with other fractions. Ethanol and Acetone fractions showed significant activity against EAC cell line. But Ethyl acetate fraction showed moderate activity against EAC cell line. The anticancer nature of the ethanol fraction is due to the presence of phytoconstituents like flavanoids, alkaloids, phenolic compounds, terpenoids, etc.

Keywords: Vitex pinnata Linn, MTT, EAC, L6, Cell viability.