Abstract

ASPIRIN INDUCED ANTI ULCER ACTIVITY OF COW URINE BETEL VINE EXTRACT

Dr. N. B. Sridhara Murthy*, B. A. Sowmya and Manasa

ABSTRACT

To evaluate the Anti-ulcer activity of Betel wine with Cow urine by Aspirin induced ulcer model in rats. The leaves of Piper betel was dried under shade and then powdered and extracted with cow urine by maceration process and the resultant extract was subjected for phytochemical analysis to identify different phytoconstituents. An acute toxicity study of the extract was done up to 5000mg/kg according to OECD guidelines No 425. Histopathological changes of stomach were assessed for confirmation. Phytochemical investigation of Cow urine Betel vine extract revealed the presence of alkaloids, glycosides, flavonoids, saponins, carbohydrates, proteins, oils and fats. In acute toxicity studies Cow urine extract up to dose level of 5000mg/kg b.w did not revealed any variation in their physical observation or mortality, which indicates their nontoxic nature practically and safety of the extract. Based on the toxicity studies 250 and 500 mg/kg b.w was considered as the therapeutic dose as per the OECD guide lines. Oral administration of Cow urine Betel vine extract at the selected therapeutic doses showed dose related antiulcer activity in Aspirin induced ulcer model. The histopathological examination of the negative control treated stomach shows severe erosions of gastric mucosa, sub mucosal edema and neutrophil infiltration, which were absent in the extract and standard treated group.

Keywords: Piper betel, peptic ulcer, Aspirin.