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COMPARATIVE HPTLC ANALYSIS OF COCOS NUCIFERA LINN PERICARP SUCCESSIVE EXTRACT AND OIL*Borhade Pravin Sopan1, Dalal Preeti Satish2, Pachuri Asawari D2, Lone Krishnakumar Devrao3, Patole Vinita Chandrakant3, Bhingole Santoshkumar Venkatreddy3.
1Department of Pharmacognosy, JSPM’s Rajarshi Shahu College of Pharmacy and Research, Tathawade, Pune, Maharashtra, India. ABSTRACT Aim: High performance thin layer liquid chromatography (HPTLC) was more popular in quality control and standardization of traditional herbs. Keeping in view of this goal of the present study to develop chromatography fingerprinting of successive extracts of Cocos nucifera fruit pericarp and compare this with oil obtained by combustion method. Material and method: Cocos nucifera Linn belong to family Arecaceae widely growing palm in southern Kerala region of India. Dried pericarp powder was subject to continuous hot soxhlet extraction method using petroleum ether, chloroform and methanol. Combustion method was use to obtained coconut pericarp oil. Chromatographic separation was achieved on a pre-coated with silica gel 60 F254 plate using a mixture of benzene: ethyl acetate (9.0: 1.0 v/v) as a mobile phase at a wavelength of 254 nm. Result: HPTLC chromatographic separation of pericarp oil and successive petroleum ether, chloroform and methanol shows that numbers of phytoconstituents present in oil are more than that of extract. Total number of constituents observed in chromatograph of oil was 10 while petroleum ether, chloroform and methanol extracts shows 4, 6 and 7 constituents respectively. Constituent No 1 was present in all but its concentration is more in oil as compare to extracts. Constituent No 9 was only present in oil chromatograph and absent in all successive extracts. Keywords: Arecaceae, Cocos nucifera, Chromatography, Physicochemical. [Download Article] [Download Certifiate] |
