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  • SEPTEMBER 2021 Issue has been successfully launched on 1 September 2021.

Abstract

AN IN VITRO ANTICANCER ACTIVITY OF AQUEOUS EXTRACT OF TRITICUM AESTIVUM ON HEP2 HUMAN EPITHELIOMA OF LARYNX CELL LINE.

Dr. Janki B. Patel*

ABSTRACT

The study was aimed to evaluation of anticancer activity of the leaves of Triticum aestivum on Hep‐2 cell line from human epithelioma of larynx. The aqueous extract of leaves of Triticum aestivum was tested for its inhibitory effect on Hep‐2 cell line in comparison with the Vero (normal kidney cells) cell line. The cytotoxic activity was investigated in-vitro using 3‐(4, 5‐dimethyl thiazol‐2yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay. The potency of each plant extract concentration was calculated in terms of percent cell inhibition of HEp‐2 cells as compared to the Vero (normal kidney cells) cell line. The extract showed dose dependent antitumor activity. The MTT assay showed an anti proliferative activity (IC50) for the HEp- 2 cell line at 335.3 μg/ml of crude extract.

Keywords: Triticum aestivum, HEp?2 cells, Vero cell line, MTT assay, Cytotoxicity.


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