Photo Gallery

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from  7.454 to 7.632  due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • WJPPS: SEPTEMBER ISSUE PUBLISHED
  • SEPTEMBER 2021 Issue has been successfully launched on 1 September 2021.

Abstract

STUDYING THE CLONING AND EXPRESSION OF ?-GLUCOSIDASE USING YAKULT AND STRAWBERRY PROBIOTICS AS POTENTIAL SOURCE OF BIFIDBACTERIUMBREVE

P. Singh*, A.N. Singh and Madhulika Singh

ABSTRACT

Bifidobacteriumbreve is important for food industries since it is known to be a great source of β-glucosidase which helps in lactose digestion. In the present study the samples of Strawberry and Yakult were collected for the isolation of bacterial species using spreading and streaking techniques. The isolated bacterial species were identified by performing biochemical tests. Genomic DNA from B.breve was cleaved with the restriction enzyme SmaI andβ-glucosidase geneisligated to pBR322 for transformation into Escherichia coliDH5 α. β-glucosidase gene was functionally expressed in E.coli DH5 α; the transformants were able to grow on ampicillin.β-glucosidase positive clone was demonstrated in the results by presence of its colonies on a medium supplemented with ampicillin.

Keywords: ?-glucosidase, Probiotics, Bifidobacteriabreve and Escherichia coli DH5 ?.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More