WJPPS Citation

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • New Impact Factor
  • WJPPS Impact Factor has been Increased to 8.025 for Year 2024.

  • WJPPS: MARCH ISSUE PUBLISHED
  • March Issue has been successfully launched on 1 March 2024.

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • Scope Indexed
  • WJPPS is indexed in Scope Database based on the recommendation of the Content Selection Committee (CSC).

Abstract

MACROSCOPIC AND MICROSCOPIC CHARACHTERIZATION OF ATRIPLEX HALIMUS L. GROWING IN EGYPT AND IN VITRO EVALUATION OF ITS CYTOTOXIC ACTIVITY

Amal M. Kabbash*

ABSTRACT

Herbal medicines have been commonly used and remain so instead of synthetic drugs because of their possible fewer side effects. Atriplex halimus L. (Amaranthaceae), denominated saltbush, is a promising medicinal plant growing in Egypt. The aim of this study is to address the botanical identification of this valuable plant and evaluate its cytotoxic activity. Transverse sections and macerated tissues were examined. From present evaluation, it is evident that certain characters such as hair, stomata in epidermal layer, calcium oxalate, vascular bundles and xylem vessels can provide useful information for correct identification of the plant. In addition, quantitative measurement of different cells dimensions is presented as an evidence for correct identification using Orion software version 6. Cytotoxicity (SRB assay) of the plant total extract and different fractions was assessed using cells from human hepatocellular (HepG2), colon (HCT), intestinal (CACO), larynx (HEP2), cervical (HELA), lung (A549), breast (MCF-7), and prostate (PC3) carcinoma and doxorubicin was used as a standard. The results showed that the total methanol-methylene chloride (1:1) extract inhibited the survival of the different tested cell lines by 67±0.76, 75±1.7, 62.5±0.9, 73.5±1.14, 73.0±1.3, 74.5±1.76, 80.5±1.5, and 78.0±0.67 %, respectively. Furthermore, the cytotoxic activities of different fractions obtained after MCI-gel CHP20P column fractionation were evaluated. Fractions eluted with water, and H2O-MeOH (40:60) showed the highest activities against prostate and breast carcinoma cell lines with IC50 of 43.7±0.56, and 25±0.87 μg/mL, respectively. It was concluded that Atriplex halimus L. could be correctly identified using some characteristic macroscopic and microscopic features together with quantitative determination of different cells dimensions. The total extract and different fractions of A. halimus L. possess a dose dependent cytotoxic activity with specific selectivity against MCF-7 and PC3 carcinoma cells.

Keywords: Atriplex halimus L., Macroscopic, Microscopic, Cytotoxicity, MCF-7 and PC3 carcinoma cells.


[Download Article]     [Download Certifiate]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More