Abstract

OXIDATIVE STRESS CAUSED BY DIETHANOLAMINE IN LIVER OF MICE AND ITS AMELIORATION BY CURCUMIN

Hetal Doctor, Sanman Samova and R.J. Verma*

ABSTRACT

The present study was an attempt to evaluate oxidative stress caused by diethanolamine (DEA) and its amelioration by curcumin. Oral administration of DEA (110, 165, 330 mg/kg body weight/day) in mice for 30 days caused significant (p<0.05), dose-dependent increase in lipid peroxidation in the liver of mice than that of untreated control. The activities of enzymatic antioxidants (glutathione peroxidase, superoxide dismutase and catalase) as well as contents of non-enzymatic antioxidants (total ascorbic acid and glutathione) were significantly (p<0.05) reduced in the liver of DEA- treated mice as compared to untreated control group. Oral administration of curcumin (10, 20, 30 mg/kg body weight/day) along with high dose of DEA for 30 days significantly (p<0.05) ameliorates DEA- induced lipid peroxidation. This might be due to elevated levels of enzymatic and non-enzymatic antioxidants in the liver as compared to DEA alone treated mice. These findings suggests that curcumin treatment significantly (p<0.05) ameliorates DEA-induced oxidative stress in the liver of mice.

Keywords: Oxidative stress, enzymatic and non-enzymatic antioxidants, lipid peroxidation, curcumin.