Abstract

EXTRACTION OF TYROSINE AMMONIA LYASE (TAL) FROM MURRAYA KOENIGII PLANT FOR THE TREATMENT OF HYPERPIGMENTATION - INVOLVES IN-VIVO ANALYSIS ON ZEBRAFISH EMBROYS & MOLECULAR DOCKING

A.J.M. Difran, Abhinaya H.V., Sanchi Verma, Sanju Sampath1, Umme Umaima, Swetha B.N. and Guruprasad R.*

ABSTRACT

Tyrosine ammonia lyase (L-tyrosine ammonia-lyase, TAL or Tyrase, EC 4.3.1.23) is an enzyme in the natural phenols biosynthesis pathway. It is an enzyme required for deamination of the amino acid L-tyrosine to p-Coumaric acid.[1] [2] [3] [4] The enzyme TAL was extracted from the plant Murraya koenigii. This enzyme can be used in the treatment of hyperpigmentation where more amount of tyrosine gets converted to melanin, responsible for skin colour. The extraction of this enzyme was based on the principle of separation of proteins on treatment with acetone. The presence of enzyme is to be detected hence standardization of the enzyme assay was accomplished. The presence of the enzyme was confirmed by the formation of p-coumaric acid which was detected by using UV-Visible spectrophotometer at 380nm. In-vivo experimental analysis of enzyme is done on embryos of zebra fish-used as model organism. Autodock, Chemsketch, Open Babble & Pymol softwares were used for molecular docking.

Keywords: L-tyrosine ammonia-lyase, hyperpigmentation, p-coumaric, Murraya koenigii, zebrafish.