Abstract

EVALUATION OF ANTI-HELICOBACTER PYLORI (DSMZ 10242) ACTIVITY AND QUALITATIVE ANALYSIS OF QUERCETIN BY HPLC IN PHYLLANTHUS NIRURI LINN.

Baljinder Kaur*, Navneet Kaur and Vikas Gautam

ABSTRACT

Phyllanthus niruri Linn. is traditionally used in folk medicine for the treatment of a variety of ailments such as asthma, arthritis, poor appetite, constipation, cuts and bruises, corneal opacity, conjunctivitis, flu and colds, blennorrhagia, colic, diabetes, dropsy, dysentery, dyspepsia, fever, flu, gout, gonorrhea, itch, jaundice, kidney aliments, leucorrhea, malaria, menorrhagia, menstrual troubles/complaints, obesity, proctitis, stomachache, tenesmus, tumor, typhoid fever and vaginitis. The present study was aimed to evaluate the anti- Helicobacter pylori and urease inhibition activities of hydroalcoholic extracts from different regions of Punjab using simple plate assays. From the study it was reported that hydroalcoholic extract from Patiala exhibited stronger anti-H. pylori activity (20 mm) than extracts from other regions of Punjab. Moreover, quercetin was determined in all the extracts using HPLC and maximum concentration of quercetin was also found in extract from Patiala region (574.8 mg/g). Preliminary studies revealed that the inhibitory mechanism may involve quercetin based noncompetitive urease inhibition.

Keywords: Anti-Helicobacter pylori, Phyllanthus niruri Linn., Urease inhibition, Quercetin, HPLC, Anti-microbial activity.