Photo Gallery

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from  7.454 to 7.632  due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • WJPPS: SEPTEMBER ISSUE PUBLISHED
  • SEPTEMBER 2021 Issue has been successfully launched on 1 September 2021.

Abstract

METHOD DEVELOPMENT AND VALIDATION OF BOSENTAN IN BULK AND PHARMACEUTICAL FORMULATION BY USING HPTLC

Lakshmi Madhuri B., Savita Yadav, Chaitali Dhale and Janhavi Rao*

ABSTRACT

In the present study a rapid specific economical HPTLC method has been developed for determination of Bosentan in bulk and pharmaceutical dosage formulation. Chromatographic separation was achieved on aluminum plates precoated with silica gel 60 F254 aluminum plates with mobile phase of methanol: toluene in the ratio of 2: 8(v/v). Detection was performed densitometrically at 225 nm using Camag TLC Scanner. The Rf of Bosentan found to be 0.32.The Method was validated as per ICH guidelines in terms of linearity (40- 400 ng/band), precision (intra-day variation 0.4, inter-day variation 0.3), accuracy (99.04 to100.6%) and specificity. The limit of detection and limit of quantification for Bosentan were found to be 10 ng/spot and 30 ng/spot, respectively. The method can be used as routine analysis of Bosentan in pharmaceutical formulation.

Keywords: Thin layer chromatography, Densitometry, Validation, Bosentan.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More