Abstract

EFFECTIVE VACCINE DEVELOPMENT AGAINST STAPHYLOCOCCOSIS FROM STAPHYLOCOCCUS AUREUS

S. Mumtaj*, G. B. Brindha Devi, P. Rajalakshmi and S. Sheela

ABSTRACT

Staphylococcus aureus causes Staphylococcosis in cultured and wild fish populations, implicated by exophthalmia and swollen lesion on the tail, often causing high mortality. Aquaculture has grown at an average rate of 8.9 percent per year since 1970 and has become the fastest growing sector of food production in the world. Fish vaccination with a safe and effective vaccine is a potential approach for prevention and control of fish disease. The main objective of the current study was to develop WC [Whole Cell-Formalin-Killed] and OMP [Outer membrane Protein] vaccines from Staphylococcus aureus against staphylococcosis. OMP and WC Vaccines were prepared from MTCC 96 strain of Staphylococcus aureus. Prepared Vaccines were resolved on a 10% SDS-PAGE, to generate protein profiles and it was quantified following the protocol of Lowry et al., SDS-PAGE results showed that the WC vaccines had ten polypeptide bands with molecular weight of 161.2, 137.1, 112.1, 85.8, 73.4, 63.1, 55.8, 46.2, 36.1 and 33.1 KDa and OMP vaccines had four Polypeptide band with molecular weight of 84.9, 72.2, 60.5, and 46.9 KDa. The Protein concentration for WC and OMP vaccines was estimated as 88μg/ml and 55 μg/ml. The immunoproteomic vaccines prepared might prove useful in the Aquaculture Industry due to its long lasting immunity and also it will be economically effective.

Keywords: Staphylococcus aureus, WC, OMP.