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Abstract

BIOACTIVITY GUIDED ISOLATION OF CRUDE ETHANOL EXTRACT OF AZIMA TETRACANTHA LEAVES

Sundaresan Nandhini*, Ramalingam Radha and Narayanan Jayshree

ABSTRACT

Objective: To evaluate the antioxidant activity of the isolated fractions of crude ethanolic extract of Azima tetracantha. Methods: The crude ethanolic extract of Azima tetracantha leaves was subjected to chromatographic separation using column and thin layer chromatographic techniques. All the fractions were screened for antioxidant activity using DPPH assay in invitro. Results: The extract yielded 15 component fractions. From which, fraction 32 (F32) exhibited the most profound anti-oxidant activity.

Keywords: Azima tetracantha, Bioactivity guided isolation, Antioxidant, DPPH assay.


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