Abstract

SIMULTANEOUS ESTIMATION OF GLYCYRRHIZIN AND CATECHIN FROM MARKETED HERBAL FORMULATIONS BY HPTLC

Dibyangana Susanta Duttagupta*, Dr. Varsha Milind Jadhav, Dr. Vilasrao J. Kadam

ABSTRACT

The present work deals with development of HPTLC method for simultaneous estimation of glycyrrhizin and catechin in marketed herbal formulations containing Licorice root and Ashoka bark as the major ingredients. Chromatographic separation of the drugs was performed on Merck TLC aluminium plates pre-coated with silica gel 60F254 as the stationary phase. The mobile phase selected was toluene: ethyl acetate: methanol: formic acid (3.5:3.5:2:1v/v/v/v). The sample solutions were prepared in methanol and linear ascending development was carried out in twin trough glass chamber and scanned at 262 nm using Camag TLC scanner. The two markers were satisfactorily resolved with Rf values 0.28±0.02 and 0.57±0.02 for glycyrrhizin and catechin, respectively. The linear regression analysis data for the calibration plots for glycyrrhizin and catechin showed good linear relationship with regression coefficient (r2) 0.9979 and 0.9986 in the concentration range of 200-900 ng/spot and 300-1000 ng/spot, respectively. The method was validated for linearity, specificity, recovery, precision, robustness as per ICH guidelines. Limits of detection and quantitation were recorded as 16.862 and 51.099 ng/spot, respectively for glycyrrhizin, similarly 11.883 and 36.009 ng/spot, respectively for catechin. The developed method was found to be rapid, simple, precise, specific and reliable for the determination of glycyrrhizin and catechin from these marketed herbal formulations.

Keywords: Glycyrrhizin, Catechin, HPTLC, Validation, ICH guidelines.