WJPPS Citation

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • New Impact Factor
  • WJPPS Impact Factor has been Increased to 8.025 for Year 2024.

  • WJPPS: MARCH ISSUE PUBLISHED
  • March Issue has been successfully launched on 1 March 2024.

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • Scope Indexed
  • WJPPS is indexed in Scope Database based on the recommendation of the Content Selection Committee (CSC).

Abstract

SIMULTANEOUS ESTIMATION OF GLYCYRRHIZIN AND CATECHIN FROM MARKETED HERBAL FORMULATIONS BY HPTLC

Dibyangana Susanta Duttagupta*, Dr. Varsha Milind Jadhav, Dr. Vilasrao J. Kadam

ABSTRACT

The present work deals with development of HPTLC method for simultaneous estimation of glycyrrhizin and catechin in marketed herbal formulations containing Licorice root and Ashoka bark as the major ingredients. Chromatographic separation of the drugs was performed on Merck TLC aluminium plates pre-coated with silica gel 60F254 as the stationary phase. The mobile phase selected was toluene: ethyl acetate: methanol: formic acid (3.5:3.5:2:1v/v/v/v). The sample solutions were prepared in methanol and linear ascending development was carried out in twin trough glass chamber and scanned at 262 nm using Camag TLC scanner. The two markers were satisfactorily resolved with Rf values 0.28±0.02 and 0.57±0.02 for glycyrrhizin and catechin, respectively. The linear regression analysis data for the calibration plots for glycyrrhizin and catechin showed good linear relationship with regression coefficient (r2) 0.9979 and 0.9986 in the concentration range of 200-900 ng/spot and 300-1000 ng/spot, respectively. The method was validated for linearity, specificity, recovery, precision, robustness as per ICH guidelines. Limits of detection and quantitation were recorded as 16.862 and 51.099 ng/spot, respectively for glycyrrhizin, similarly 11.883 and 36.009 ng/spot, respectively for catechin. The developed method was found to be rapid, simple, precise, specific and reliable for the determination of glycyrrhizin and catechin from these marketed herbal formulations.

Keywords: Glycyrrhizin, Catechin, HPTLC, Validation, ICH guidelines.


[Download Article]     [Download Certifiate]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More