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E. Rethna Priya, S. Ravichandran* and Sukanya Ghosh


Antimicrobial peptides are small peptides existing widely in mammals, amphibians and marine invertebrates they appear to be one of the factors in innate immunity. Antimicrobial peptides are widely expressed in organisms and have been linked to innate and acquired immunities in marine vertebrates. In the present study antimicrobial and anticancer activity of the puffer fish Lagocephalus spadiceus was explored. Antimicrobial screening was done in ten consecutive human bacterial and fungal pathogens by using standard disc diffusion method and the anticancer profile was done using MTT assay. In the antibacterial the maximum zone of inhibition was observed against V. cholera. In the antifungal activity the maximum zone of inhibition was observed against A. terreus. The present study clearly shows that the various fractions was found to be rich in ninhydrin positive spots indicating the possibility of containing peptides, further confirmed by FTIR and the presence of doublets in the region of its NMR spectrum. Hence the present study indicated that the puffer fish L. spadiceus may have potential antibiotics which can be useful for human welfare.

Keywords: Puffer fish, Hemolymph, Anti-microbial Peptide, TLC, NMR.

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