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Abstract

A SIMPLE VALIDATED RP- HPLC METHOD FOR THE ANALYSIS OF FLUCLOXACILLIN SODIUM IN CAPSULE DOSAGE FORM

Michael Worlako Klu*, Bright Selorm Addy and David Ntinagyei Mintah

ABSTRACT

A simple, cheap, fast and accurate reverse phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for the assay of flucloxacillin sodium in capsule dosage form. The method was developed using a Phenomenex ® Bondclone 10 C18 column (300×3.9 mm, 5μm) and a mobile phase system made of 60% Methanol and 40% KH2PO4 buffer adjusted to a pH of 5 with 1M sodium hydroxide solution. The method was validated and it was found to be linear, selective, accurate, robust and precise. The limit of detection (LOD) and limit of quantification (LOQ) respectively were 0.00437% w/v and 0.0132% w/v. The method is suitable for routine assay of flucloxacillin sodium in capsule dosage form because it is simple, cheap and accurate.

Keywords: Flucloxacillin sodium, RP- HPLC, assay.


[Full Text Article]

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