Photo Gallery

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from  7.454 to 7.632  due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • WJPPS: SEPTEMBER ISSUE PUBLISHED
  • SEPTEMBER 2021 Issue has been successfully launched on 1 September 2021.

Abstract

EVALUATION OF THE INTERACTION BETWEEN AZITHROMYCIN AND HUMAN SERUM ALBUMIN BASED ON Ru(bpy)3 2+-DOPED SILICA NANOPARTICLES PROBE

Jianlin Zhu, Ming Su* andHanwen Sun

ABSTRACT

The interaction between azithromycin and human serum albumin (HSA) was investigated by using capillary electrophoresis (CE) coupled with end-column solid-state electrochemiluminescence (ECL). Ru(bpy)3 2+-doped silica nanoparticles (RuDS) were prepared by an one-pot water-in-oil microemulsion method. The as-prepared RuDS was displayed ECL signal at around 1.18 V. The ECL signal was further increased in the presence of azithromycin, which could be used for end-column detection of azithromycin concentration. Under the optimized condition, the ECL intensity was linear with azithromycin concentration in the range of 0.1 to 140 μmol/L (R2=0.9983) with a detection limit of 0.05 μmol/L. Based on this, the binding constant between azithromycin and HSA was estimated to be 7.46×104 L/mol by nonlinear regression analysis assuming that only one special binding site for drug coupling provided per protein. Warfarin and ketoprofen were used as probes to study their interaction mechanism and the result showed that azithromycin binds at warfarin site (site I) of HSA. The proposed CE-ECL method may be applicable to study a wide range of interactions between drugs and biomacromolecules such as proteins and DNA.

Keywords: Capillary electrophoresis; Electrochemiluminescence; Azithromycin; Human serum albumin; Interaction.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More