QUANTITATIVE SPECTROPHOTOMETRIC DETERMINATION OF ARTESUNATE IN PHARMACEUTICALS USING THE IRONTHIOCYANATE COMPLEX FORMATION REACTION
Attih Emmanuel*, Usifoh C. O. Udobang John, Uwa Timma
ABSTRACT
A sensitive, accurate and reproducible spectrophotometric method is
developed for the elimination of Artesunate in pharmaceutical
formulations. This novel method is based on the oxidation of FeII to
FeIII by artesunate in acid medium and the subsequent formation of
Ferric-thiocynate complex (blood red) chromogen which absorbs, Uvvis
light maximally at 480nm. Under optimized experimental
conditions, the absorbance was found to increase linearly with the
artesunate concentration. A calibration curve was generated which
obeyed beer’s law within the range of 0.4 – 50 μg/ml with a linear coefficient
of 9997, a slope of 0.0113 and a negligible intercept. The
molar absorptivity and Sandell sensitivity were 1.08x104 LMol-1 CM-1
and 0.0356 μg/cm respectively. The limit of detection (LOD) and
Limit of Qualification (LOQ) were 0.15 and 1.29 μg/ml respectively. The accuracy expressed
as percentage relate error (ER%) and the corresponding precision expressed as relative
standard deviation (RSD %) were <3.0% and <2.5% respectively. The proposed method was
validated and used to assay artesunate in tablet formulations procured locally, the result
obtained was statistically compared with official international pharmacopoeia method for
artesunate; the result showing no difference in their means. The t and f value were <1.50 and
> 2.76 respectively. The accuracy and practicability of the method was ascertained by recovery studies performed via standard addition method with the results showing high
accuracy and virtually no interference from pharmaceutical excipients.
Keywords: Artesunate, Counterfeit, Malaria, Pharmaceutical, Spectrophotometry.
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