Abstract

CYTOTOXIC EFFECT OF CAMELLIA SINENSIS ON THE NORMAL MYELOID AND CANCER CELLS OF PATIENTS WITH CHRONIC MYELOGENOUS LEUKEMIA

Prof Al –Jawad F.H., Prof. Yaseen N.Y. and *Inssaf Al- Shemmary

ABSTRACT

Objective: The present study was designed to determine in vitro the cytotoxic effect of green tea on the normal and leukemic myeloidstem cells of patients with chronic myelogenous leukemia. Methods: the serial dilution of aqueous green tea extraction in the concentrations (250, 125, 62.5, 31.25, 15.625,7.8125 μg/ml ) were added to the cells culture. Using MTT assay. Results: the results show the cytotoxic effect (inhibition rate of growth) of green tea (viable cell count was 52x105) was in dose and time dependent. There were significant differences (P≤0.05) between concentrations and time. The highest inhibition rate on the leukemic cells was 71.283% in concentration 250μg/ml and the lowest was 38.370% in concentration 7.8125 μg/ml for exposure time 72hr While the highest inhibition rate on the normal myeloid cells was 35.772% inconcentration 250μg/ml and the lowest was 11.016% in concentration 7.8125 μg/ml for exposure time 72hr. Conclusions: Green tea showed high effect on the leukemic cells and less effect on the normal myeloid cells culture.

Keywords: Green tea extraction, normal and leukemic myeloid cells.