Abstract

ANALGESIC AND ANTI-INFLAMMATORY ACTIVITY OF NOVEL (FIXED DOSE) TOPICAL GEL CONTAINING DICLOFENAC COMBINATION WITH HEPARIN

J. Balasubramanian*, K. Sai Sugathri, K. Jothi, G.A. Nandhini and S. Hariram

ABSTRACT

Objective: The aim of the present research work is to validate analgesic and anti-inflammatory activity of Novel (Fixed Dose) Topical gel containing Diclofenac Combination with Heparin. Methods: In the current study rodents were used to demonstrate the analgesic and anti-inflammatory properties of Diclofenac combination with heparin, in investigation of therapeutic treatment for diseases with pain and inflammation. Wister albino rats are used in paw edema method and Swiss albino mice for formalin induced paw licking method. Results: The present study of formalin induced paw licking method of analgesic activity, using Swiss albino mice shows 24.68 ± 2.5 in Ist phase of the study in between 0-5 minutes of interval compared to Std as 26.81 ± 3.14. In second phase the test formulation gives 6.5 ± 0.48 in 15-30 minutes while comparing with the Std 7.2 ±1.3. In anti- inflammatory activity of Wister albino rats for Carrageenan induced Paw edema method results in 95% in 2 hrs and 100% in 4 hrs while comparing with Std. 77% and 93%. Conclusion: The results of the current study reveal that the combination of diclofenac with heparin Fixed dose combination proves significant analgesic and antiinflammatory activity.

Keywords: Diclofenac sodium, Heparin sodium, Analgesic effect, Anti-inflammatory, Paw licking method, Carrageenan induced Paw edema method.