Abstract

NEW SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF SULFAMETHOXAZOLE DRUG

Mohauman Mohammad Al-Rufaie*

ABSTRACT

A simple, rapid and sensitive Spectrophotometric method for the determination of microgram amounts of Sulfamethoxazole(SMZ) drug as bluk and in diluted solution is described. The method is based on the azo-coupling reaction between Sulfamethoxazole and(4-amino-2- Hydroxy acetophenon )(SDA) to form an intense colored product with maximum absorption at 424 nm. Beer’s law is obeyed over the concentration range of( 0.5 – 18) ppm with molar absorptivity of 2.5760 x104 l.mol-1.cm-1 and Sandell`s sensitivity of 0.009 μg.cm-2 . the detection limit (LOD) was 0.476 μg/ml-1 . LOQ was 0.289 μg/ml-1. The method does not resort to solvent extraction. The optimum conditions for all colour development are described and the proposed method has been successfully applied for the determination of quantities of Sulfamethoxazole in bulk drug and pharmaceutical Preparations by using initial rate method and fixed time methed. The common excipients and additives did not interfere in this method.

Keywords: Sulfamethoxazole, azo-coupling Kinetic Spectrophotometric method.