Abstract

EXTRACTION OF TYROSINE AMMONIA LYASE (TAL) FROM CYNODON DACTYLON; ITS ACTIVATORS FROM OCMIUM TENUIFLORUM, DIFFERENT METAL IONS FOR THE TREATMENT OF HYPER PIGMENTATION AND THE EFFECT OF METAL TOXICITY.

Aishwarya H., Nidhi Mohan, Bhargav K. M. and Guruprasad R.*

ABSTRACT

Hyper Pigmentation is a common problem in humans due to increased melanin production. L-Tyrosine in melanocytes gets converted to melanin via Biosynthetic pathway. Tyrosine Ammonia Lyase [TAL; E.C.4.3.1.23] prevents melanin formation by breaking down LTyrosine to p-coumaric acid and ammonia. It is extracted from Cynodon dactylon and some quantity of crude enzyme is treated with metal compounds, ethanol extract of Ocmium tenuiflorum to form enzyme metal complex and Ocmium tenuiflorum + Enzyme extract respectively. Zebrafish is an important model organism to understand how genes work in human health and disease. Zebrafish embryos are treated with crude enzyme, metal enzyme complex, Ocmium tenuiflorum + Enzyme extract and their effects on reducing pigmentation are compared. Though the metal enzyme complex has more enzymatic activity than crude enzyme, it leads to metal toxicity and Zebrafish die instantly. By Insilco method (autodock) , “4-(3-hydroxy-1-propenyl)-2-methoxy Phenol” compound from ethanol extract is found to be most probable to form a compound with crude enzyme as it enhances enzymatic activity and nullifies pigmentation.

Keywords: Cynodon dactylon, Ocmium tenuiflorum.