WJPPS Citation

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • New Impact Factor
  • WJPPS Impact Factor has been Increased to 8.025 for Year 2024.

  • WJPPS: MARCH ISSUE PUBLISHED
  • March Issue has been successfully launched on 1 March 2024.

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • Scope Indexed
  • WJPPS is indexed in Scope Database based on the recommendation of the Content Selection Committee (CSC).

Abstract

CELLULASE PRODUCTION AND OPTIMIZATION OF PROCESS PARAMETERS BY ONE FACTOR AT A TIME TECHNIQUE FROM MUTANT STRAIN OF BACILLUS STRATOSPHERICUS N12 (M) AND ITS SCALE UP IN A STIRRED TANK BIOREACTOR

Nisha Sharma* and Nivedita Sharma

ABSTRACT

In the present study, enhanced production of cellulase by a stable ethidium bromide treated mutant strain of Bacillus stratosphericus N12 (M) by optimizing various process parameters using one factor at a time (OFAT) technique and scale up in a stirred tank bioreactor has been done. The ethidium bromide mutated strain of B. stratosphericus N12 (M) has shown an increased cellulase production over the wild type strain of B. stratosphericus N12 (W). A good stability until 8 generations has also been observed. Optimization of process parameters by OFAT for enhanced enzyme production yielded 5.983 IU of enzyme on PYC medium at 8.0 pH with 10.0% inoculum following 72 h of incubation time. Further scale up of enzyme production in a 7.5 L stirred tank bioreactor, utilizing the optimized conditions yielded 2.443 IU after 8 h of fermentation thus proving the efficiency of a stirred tank bioreactor in enzyme production with higher volumes in short time.

Keywords: Bacillus stratosphericus, cellulase, mutation, ethidium bromide, bioreactor.


[Download Article]     [Download Certifiate]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More