Abstract

BENEFICIAL EFFECT OF TRIGONELLINE ON THE METABOLIC CHANGES ASSOCIATED WITH INSULIN RESISTANCE IN RATS

Amer A. Ramadan, Nehal A. Afifi*, Emad Y.Erian, Dalia O. Saleh, Ahmed A. Sedik

ABSTRACT

The objective of the present study was to evaluate the protective effect of trigonelline (TRIG) on the high fat high fructose (HFHF) - induced insulin resistance in rats. Insulin resistance was induced by adding high-fat diet (60 kcal/100 kcal saturated fat) and 10% fructose in the drinking water to rats for 8 weeks. Insulin resistant rats were orally treated with both TRIG (50 and 100 mg/kg b.wt) and Sitagliptin (SITA; 10 mg/kg b.wt) for 14 consecutive days. Twenty-four hours after the last dose of the drugs, the 18 hours fasting rats were weighed; and their sera was assayed for blood glucose, serum insulin, total cholesterol (TC) and triglyceride (TG) levels. TRIG significantly decreases blood glucose, TC and TG levels of insulin resistant rats. Liver to body weight ratio and insulin level were altered in insulin resistant rats and were near control levels treated with TRIG. Conclusion: The obtained findings suggested that trigonelline may have beneficial hypoglycemic effect due to its protective effects on β-cells and antioxidant potential which was on insulin resistant rats.

Keywords: Trigonelline - high fat high fructose- insulin resistance- rats.